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Silica Surface Characterization as a Function of Formation and Surface Treatment Using Traditional Methods and Proteins as Surface Probes

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dc.contributor.advisor Clare, Alexis Korwin-Edson, Michelle 2017-02-07T15:25:47Z 2017-02-07T15:25:47Z 2004-05
dc.description Advisory committee members: Thomas Seward, Alan Goldstein, William Lacourse. Dissertation completed in partial fulfillment of the requirements for the degree of Doctorate of Philosophy in Glass Science at the Kazuo Inamori School of Engineering, New York State College of Ceramics at Alfred University en_US
dc.description.abstract The motivation behind this work stems from Jedlicka’s work on Chinese hamster ovary (CHO) cells and her observation that these cells proliferated differently depending on the glass chemistry on which the cells were growing. It is well established that proteins form the bonds between cells and glass substrates and so this work was aimed at discovering whether proteins also react differently to different glass surfaces. It is believed that the bond formed between glass and protein is a hydroxyl-amine interaction via hydrogen bonding. The scope of this work deals with silica glass in various forms including slides, cane, fiber, micron-sized spheres and Cab-o-Sil®. These forms are subjected to surface treatments such as ethanol cleaning, HF acid etching, water plasma treatments and 1000°C thermal treatments. A select few proteins are chosen as a tool to probe the surfaces of the silica glass and single crystal quartz surfaces. These proteins are human serum albumin (HSA), streptavidin, mouse immunoglobulin G (IgG), biotin, and anti-mouse IgG. A few characterization techniques are employed in an attempt to examine protein adsorption and its feasibility as a surface probe. These techniques include sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), bicinchoninic acid (BCA) assay, glancing incidence X-ray analysis (GIXA), fluorescence spectrometry, atomic force microscopy (AFM), and chemical force microscopy (CFM). The main goal of this work is to determine which of the above techniques in conjunction with protein adsorption is the most promising as a surface characterization technique. It was determined that CFM is the most promising surface characterization technique utilizing proteins as surface probes. It is possible to attach a wide variety of molecules to a standard contact mode tip including proteins. Streptavidin, –COOH, and –CH3 functionalized tips were utilized in the CFM study. The overall adhesion forces between silica and tip were greatest for –COOH, then streptavidin and least for –CH3. This ordering is a broad generalization however, since the adhesion forces depend greatly on surface treatment and can either be very consistent across the surface or vary significantly. SDS-PAGE on streptavidin was preventatively difficult, but IgG electrophoresis was possible and did show some promising results. BCA assay and fluorimetry both utilized “depleted” protein solutions and thus it was very difficult to uncover trends in the data. GIXA showed that the protein layer thickness was monolayer in nature. AFM allowed proteins to be imaged while in the tris-buffered saline and the features were on the order of ten protein agglomerations. The GIXA and AFM data agree very well. CFM is able to discern between the various surface treatments. With the eventual development of carbon nanotube growth onto a contact mode tip and the subsequent application of a single chemical molecule onto the end of the tube, CFM will become an even stronger technique for surface characterization. en_US
dc.format.extent 295 pages en_US
dc.language.iso en_US en_US
dc.publisher New York State College of Ceramics at Alfred University. Kazuo Inamori School of Engineering. en_US
dc.relation.ispartof Scholes Library en_US
dc.rights.uri en_US
dc.title Silica Surface Characterization as a Function of Formation and Surface Treatment Using Traditional Methods and Proteins as Surface Probes en_US
dc.type Thesis en_US

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