Protein Adsorption to Multi-Component Glasses
Date
2003-05
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
New York State College of Ceramics at Alfred University. Kazuo Inamori School of Engineering.
Abstract
The adsorption of human serum albumin (HSA) to sodium silicate, soda lime
silicate (SLS), and sodium aluminosilicate (SAS) glass microspheres was investigated
using sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in
conjunction with a colloidal silver stain for visualization. The 30 Na2O·70 SiO2
composition could not be evaluated due to an apparent chemical interference that
occurred during silver staining. This inhibitory effect was attributed to the extensive
corrosion that occurred during the protein elution and caused an elevation in the pH of
the solution. The remaining glass compositions were sufficiently durable for further
study.
The HSA adsorption capacity of SLS glass microspheres containing 70 and 80
mol% SiO2 increased as CaO was substituted for Na2O. An abrupt decrease in the HSA
adsorption capacity was observed for SLS glasses containing 60 mol% SiO2. A similar
trend was observed for the SAS glass microspheres, although the SAS glasses adsorbed
less HSA than the SLS glasses containing equivalent molar percentages of SiO2. The
initial increase in HSA adsorption capacity for SLS and SAS glasses containing 70 and
80 mol% SiO2 was attributed to the introduction of positive charges into the glass
surfaces via Ca2+ and Al3+ cations. The decrease in HSA adsorption capacity for SLS and
SAS glasses containing 60 mol% SiO2 may be due to an enhanced affinity between the
glasses and HSA, resulting in a “flattened” conformation that limits the total accessible
area for adsorption.
Description
Advisory committee members: Alan Goldstein, Alastair Cormack, William Lacourse, Walter Schulze. Dissertation completed in partial fulfillment of the requirements for the degree of Doctorate of Philosophy in Glass Science at the Kazuo Inamori School of Engineering, New York State College of Ceramics at Alfred University
Type
Thesis